Part:BBa_J63009

Low copy protein fusion vector (Silver lab standard)

Silver Lab alias V0002. This is a modified biobrick vector (fusion brick vector) that contains the specified EcoRI, SpeI, XbaI, and PstI sites. The only difference is that the part sequence immediately follows the XbaI site and immediately precedes the SpeI site. This change allows in-frame fusions of protein coding regions to be made using standard biobricks assembly methods.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2057
  Illegal XbaI site found at 2072
  Illegal SpeI site found at 1
  Illegal PstI site found at 15
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2057
  Illegal SpeI site found at 1
  Illegal PstI site found at 15
  Illegal NotI site found at 8
  Illegal NotI site found at 2063
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2057
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Illegal EcoRI site found at 2057
  Illegal XbaI site found at 2072
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2057
  Illegal XbaI site found at 2072
  Illegal SpeI site found at 1
  Illegal PstI site found at 15
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1096